residual DNA detection

The detection and quantification of minute amounts of residual host cell DNA can be accomplished using any one of three highly sensitive, validated methods. These methods encompass the entire range of residual DNA from nonspecific detection of total DNA to highly specific detection of single target sequences. The methods used are:

• The Threshold^TM System from Molecular Devices^® uses DNA binding proteins with high affinity for single-stranded DNA for nonspecific quantification of total DNA.
• A hybridization-based method for the detection of the specific DNA of defined origin with dot blots and hybridization of radioisotope-labeled DNA probes uses random hexamers to generate representative probes covering the whole genome of the host cells.
• A quantitative PCR-based method for the detection of specific DNA of defined origin targets a specific gene sequence for amplification and is calibrated using purified, species-matched, genomic DNA.